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MbPA

This is the MbPA project.

This is an R package for peak alignment on GCxGC-MS Data. MbPA can align homogeneous peaks.

Abstract

Background: Comprehensive two-dimensional gas chromatography time-of-flight mass spectrometry (GCxGC/TOF-MS) has been used for metabolite profiling in metabolomics. However, there is still much experimental variation to be controlled including both within-experiment and between-experiment variation. For efficient analysis, an ideal peak alignment method to deal with such variations is in great need.

Results: Using experimental data of a mixture of metabolite standards, we demonstrated that our method has better performance than other existing method which is not model-based. We then applied our method to the data generated from the plasma of a rat, which also demonstrates good performance of our model.

Conclusions: We developed a model-based peak alignment method to process both homogeneous and heterogeneous experimental data. The unique feature of our method is the only model-based peak alignment method coupled with metabolite identification in an unified framework. Through the comparison with other existing method, we demonstrated that our method has better performance.

Software: upon request

Reference

Jeong, J.; Shi, X.; Zhang, X.; Kim, S.; Shen, C. 2012. Model-based peak alignment of metabolomic profiling from comprehensive two-dimensional gas chromatography mass spectrometry. BMC Bioinformatics, 13:27.